Our lab studies how gene expression is post-transcriptionally dysregulated in various cancers, leading to generation and progression of malignancy. In particular, we are interested in RNA Binding Proteins (RBPs) that play a role in cancer development and progression by controlling localisation, stability, and translation of different functional categories of transcripts:
We use various omics technologies to study the role of RBPs in different aspects of post-transcriptional gene expression regulation. In particular, we are experts in Mass Spectrometry (MS) based proteomics, using various MS techniques to quantitatively study processes such as protein translation and RNA-Protein interactions, as well as combining proteomics data with RNA-sequencing to reveal the intricacies of post-transcriptional regulation by RBPs.
• Azman MS, Dodel M, Capraro F, Faraway R, Dermit M, Fan W, Ule J, Mardakheh FK*. (2021) An RNA-binding switch drives ribosome biogenesis and tumorigenesis downstream of RAS oncogene. BioRxiv (https://doi.org/10.1101/2021.12.16.472890).
• Dermit M
, Dodel M , Lee FCY, Azman MS
, Schwenzer H, Jones JL, Blagden
SP, Ule J, Mardakheh FK*. (2020) Subcellular mRNA localization regulates
ribosome biogenesis in migrating cells. Developmental Cell. 55(3):298-313.
• Johnny Yu, Albertas Navickas, Hosseinali Asgharian, Bruce Culbertson, Lisa Fish, Kristle Garcia, John Paolo Olegario, Maria Dermit, Martin Dodel, Benjamin Hanisch, Yikai Luo, Ethan M Weinberg, Rodrigo Dienstmann, Robert S Warren, Faraz K Mardakheh and Hani Goodarzi. (2020) RBMS1 suppresses colon cancer metastasis through targeted stabilization of its mRNA regulon. Cancer Discovery 10:1410–23.
Dermit M, Dodel M, Mardakheh FK*. (2017) Methods
for monitoring and measurement of protein translation in time and space. Mol Biosyst. 13:2477-2488.
Mardakheh FK*, Paul A, Kümper S, Sadok A, Paterson
H, Mccarthy A, Yuan Y, and Marshall
CJ. (2015) Global
analysis of mRNA, translation, and protein localizations: local translation is a key regulator of cell-protrusions. Developmental Cell. 35:344-57.