Our lab studies how gene expression is post-transcriptionally is dysregulated in various cancers, leading to generation and progression of malignancy. In particular, we are interested in RNA Binding Proteins (RBPs) that play a role in cancer development and progression by controlling localisation, stability, and translation of different functional categories of transcripts:
We use various omics technologies to study the role of RBPs in different aspects of post-transcriptional gene expression regulation. In particular, we are experts in Mass Spectrometry (MS) based proteomics, using various MS techniques to quantitatively study processes such as protein translation and RNA-Protein interactions, as well as combining proteomics data with RNA-sequencing to reveal the intricacies of post-transcriptional regulation by RBPs.
• Dermit M , Dodel M , Lee FCY, Azman MS , Schwenzer H, Jones JL, Blagden SP, Ule J, Mardakheh FK*. (2019) Subcellular mRNA localization regulates ribosome biogenesis in migrating cells. bioRxiv (https://doi.org/10.1101/829739)
• Dermit M, Dodel M, Mardakheh FK*. (2017) Methods for monitoring and measurement of protein translation in time and space. Mol Biosyst. 13:2477-2488.
Mardakheh FK*, Sailem
HZ, Kümper S, Tape CJ, McCully RR, Paul A, Anjomani-Virmouni S, Jørgensen C, Poulogiannis
G, Marshall CJ, Bakal C. (2016) Proteomics profiling of interactome dynamics by
colocalisation analysis (COLA). Mol Biosyst. 13:92-105.
Mardakheh FK*, Paul A, Kümper S, Sadok A, Paterson
H, Mccarthy A, Yuan Y, and Marshall
CJ. (2015) Global
analysis of mRNA, translation, and protein localizations: local translation is a key regulator of cell-protrusions. Developmental Cell. 35:344-57.